Untargeted metabolomic analysis of human milk from healthy mothers reveals drivers of metabolite variability.

ABSTRACT: Understanding the human milk metabolome can help inform infant nutrition and health. Untargeted metabolomics was used to study breast milk from 31 healthy participants to assess the shared metabolites in milk from participants with various backgrounds and understand how different demographic, health, and environmental factors impact the milk metabolome. Breast milk samples were analyzed by four separate UPLC-MS/MS methods. Metabolite Set Enrichment Analysis was used to study the most and least variable metabolites. The associations between participant factors and the metabolome were assessed with redundancy analyses. Among all 31 participants and between each untargeted UPLC-MS/MS method, 731 metabolites were detected, of which 389 were shared among all participants. Of the shared metaboli

SUBMITTER: Holmes ZC 

PROVIDER: S-EPMC11379717 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature