Metabolic engineering of Saccharomyces cerevisiae for enhanced taxadiene production.

ABSTRACT:

Background

Metabolic engineering enables the sustainable and cost-efficient production of complex chemicals. Efficient production of terpenes in Saccharomyces cerevisiae can be achieved by recruiting an intermediate of the mevalonate pathway. The present study aimed to evaluate the engineering strategies of S. cerevisiae for the production of taxadiene, a precursor of taxol, an antineoplastic drug.

Result

SCIGS22a, a previously engineered strain with modifications in the mevalonate pathway (MVA), was used as a background strain. This strain was engineered to enable a high flux towards farnesyl diphosphate (FPP) and the availability of NADPH. The strain MVA was generated from SCIGS22a by overexpressing all mevalonate pathway genes. Combining the background strains with 16 dif

SUBMITTER: Karaca H 

PROVIDER: S-EPMC11380192 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature