Effects of Exocellobiohydrolase CBHA on Fermentation of Tobacco Leaves.

ABSTRACT: The quality of tobacco is directly affected by macromolecular content, fermentation is an effective method to improve biochemical properties. In this study, we utilized CBHA (cellobiohydrolase A) glycosylase, which was expressed by Pichia pastoris, as an additive for fermentation. The contents of main chemical components of tobacco leaves after fermentation were determined, and the changes of microbial community structure and abundance in tobacco leaves during fermentation were analyzed. The relationship between chemical composition and changes in microbial composition was investigated, and the function of bacteria and fungi in fermentation was predicted to identify possible metabolic pathways. After 48 h of CBHA fermentation, the contents of starch, cellulose and total nitrogen in

SUBMITTER: Xu X 

PROVIDER: S-EPMC11380505 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature