Generation of 'semi-guided' cortical organoids with complex neural oscillations.

ABSTRACT: Temporal development of neural electrophysiology follows genetic programming, similar to cellular maturation and organization during development. The emergent properties of this electrophysiological development, namely neural oscillations, can be used to characterize brain development. Recently, we utilized the innate programming encoded in the human genome to generate functionally mature cortical organoids. In brief, stem cells are suspended in culture via continuous shaking and naturally aggregate into embryoid bodies before being exposed to media formulations for neural induction, differentiation and maturation. The specific culture format, media composition and duration of exposure to these media distinguish organoid protocols and determine whether a protocol is guided or unguided towa

SUBMITTER: Fitzgerald MQ 

PROVIDER: S-EPMC11380594 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature