The common variable immunodeficiency IgM repertoire narrowly recognizes erythrocyte and platelet glycans.

ABSTRACT:

Background

Autoimmune cytopenias (AICs) regularly occur in profoundly IgG-deficient patients with common variable immunodeficiency (CVID). The isotypes, antigenic targets, and origin(s) of their disease-causing autoantibodies are unclear.

Objective

We sought to determine reactivity, clonality, and provenance of AIC-associated IgM autoantibodies in patients with CVID.

Methods

We used glycan arrays, patient erythrocytes, and platelets to determine targets of CVID IgM autoantibodies. Glycan-binding profiles were used to identify autoreactive clones across B-cell subsets, specifically circulating marginal zone (MZ) B cells, for sorting and IGH sequencing. The locations, transcriptomes, and responses of tonsillar MZ B cells to different T_H- cell subsets were det

SUBMITTER: Le Coz C 

PROVIDER: S-EPMC11380600 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature