Unraveling the structure and function of a novel SegC protein interacting with the SegAB chromosome segregation complex in Archaea.

ABSTRACT: Genome segregation is a fundamental process that preserves the genetic integrity of all organisms, but the mechanisms driving genome segregation in archaea remain enigmatic. This study delved into the unknown function of SegC (SSO0033), a novel protein thought to be involved in chromosome segregation in archaea. Using fluorescence polarization DNA binding assays, we discovered the ability of SegC to bind DNA without any sequence preference. Furthermore, we determined the crystal structure of SegC at 2.8 Å resolution, revealing the multimeric configuration and forming a large positively charged surface that can bind DNA. SegC has a tertiary structure folding similar to those of the ThDP-binding fold superfamily, but SegC shares only 5-15% sequence identity with those proteins. Unexpectedly,

SUBMITTER: Lin MG 

PROVIDER: S-EPMC11381335 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature