RESC14 and RESC8 cooperate to mediate RESC function and dynamics during trypanosome RNA editing.

ABSTRACT: Mitochondrial transcripts in Trypanosoma brucei require extensive uridine insertion/deletion RNA editing to generate translatable open reading frames. The RNA editing substrate binding complex (RESC) serves as the scaffold that coordinates the protein-protein and protein-RNA interactions during editing. RESC broadly contains two modules termed the guide RNA binding complex (GRBC) and the RNA editing mediator complex (REMC), as well as organizer proteins. How the protein and RNA components of RESC dynamically interact to facilitate editing is not well understood. Here, we examine the roles of organizer proteins, RESC8 and RESC14, in facilitating RESC dynamics. High-throughput sequencing of editing intermediates reveals an overlapping RESC8 and RESC14 function during editing progression acro

SUBMITTER: Wackowski K 

PROVIDER: S-EPMC11381364 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature