A diagnostic signature developed based on the necroptosis-related genes and its association with immune infiltration in osteosarcoma.

ABSTRACT:

Introduction

Osteosarcoma is a bone-derived malignancy that often leads to lung metastasis and death.

Material and methods

The RNA-seq data of TARGET-osteosarcoma were collected from TARGET database. GSE16088 and GSE12865 datasets of osteosarcoma x from Gene Expression Database (GEO) were donwloaded. ConsensusClusterPlus was used for molecular subtype classification. Univariate Cox and Lasso regression was employed to develop a risk model. To analyze the regulatory effects of model feature genes on the malignant phenotype of osteosarcoma cell lines, qRT-PCR, Transwell and wound healing assays were performed. The abundance of immune cell infiltration was assessed using MCP-Counter, Gene Set Enrichment Analysis (GSEA), and ESTIMATE. The Tumor Immune Dysfunction and Exclusion (

SUBMITTER: Bian Y 

PROVIDER: S-EPMC11381599 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature