Tumor-derived exosome PPP3CB induce gemcitabine resistance by regulating miR-298/STAT3 in pancreatic cancer.

ABSTRACT:

Purpose

Due to resistance to gemcitabine (GEM), patients with pancreatic cancer (PC) usually have poor prognosis and low survival rate. The purpose of our research was to explore the impact of exosome PPP3CB on GEM resistance in PC, and concurrently analyze the regulatory role of the miR-298/STAT3 signaling pathway.

Methods

Exosomes isolated from PC cells were verified by transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and western blotting (WB). The interaction between PPP3CB and miR-298 was verified using dual-luciferase reporter gene assay, followed by evaluation of cell growth and death using CCK8 assay, EdU staining, and flow cytometry.

Results

Increased PPP3CB expression was observed in GEM-resistant PC cells. Exosomes from PC cells a

SUBMITTER: Wang C 

PROVIDER: S-EPMC11381791 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature