A simplified protocol for deriving sterile, infectious murine Heligmosomoides polygyrus bakeri larvae.

ABSTRACT: Gastrointestinal helminth infection occurs within a diverse microbiome, complicating the interpretation of whether effects are caused by the parasite versus the microbial community. Here, we present a protocol for deriving sterile larvae of the murine helminth, Heligmosomoides polygyrus bakeri (H. polygyrus), providing experimental control of the microbiome. We describe steps for sterilizing with a bleach solution and developing into infectious larvae using E. coli. We then detail procedures for removing bacterial contaminants before harvesting to ensure the generation of germ-free larvae.

SUBMITTER: Blackwell KH 

PROVIDER: S-EPMC11381856 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature