N6-methyladenosine triggers renal fibrosis via enhancing translation and stability of ZEB2 mRNA.

ABSTRACT: In recent years, a surge in studies investigating N6-methyladenosine (m⁶A) modification in human diseases has occurred. However, the specific roles and mechanisms of m⁶A in kidney disease remain incompletely understood. This study revealed that m⁶A plays a positive role in regulating renal fibrosis (RF) by inducing epithelial-to-mesenchymal phenotypic transition (EMT) in renal tubular cells. Through comprehensive analyses, including m⁶A sequencing, RNA-seq, and functional studies, we confirmed the pivotal involvement of zinc finger E-box binding homeobox 2 (ZEB2) in m⁶A-mediated RF and EMT. Notably, the m⁶A-modified coding sequence of ZEB2 mRNA significantly enhances its translational elongation and mRNA stability by interac

SUBMITTER: Cai Y 

PROVIDER: S-EPMC11381876 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature