Transcriptional Isoforms of NAD⁺ kinase regulate oxidative stress resistance and melanoma metastasis.

ABSTRACT: Metastasizing cancer cells encounter a multitude of stresses throughout the metastatic cascade. Oxidative stress is known to be a major barrier for metastatic colonization, such that metastasizing cancer cells must rewire their metabolic pathways to increase their antioxidant capacity. NADPH is essential for regeneration of cellular antioxidants and several NADPH-regenerating pathways have been shown to play a role in metastasis. We have found that metastatic melanoma cells have increased levels of both NADPH and NADP⁺ suggesting increased de novo biosynthesis of NADP⁺. De novo biosynthesis of NADP⁺ occurs through a single enzymatic reaction catalyzed by NAD⁺ kinase (NADK). Here we show that different NADK isoforms are differentially expressed in

SUBMITTER: Cascio G 

PROVIDER: S-EPMC11381905 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature