Methane biohydroxylation into methanol by Methylosinus trichosporium OB3b: possible limitations and formate use during reaction.

ABSTRACT: Methane (CH₄) hydroxylation into methanol (MeOH) by methanotrophic bacteria is an attractive and sustainable approach to producing MeOH. The model strain Methylosinus trichosporium OB3b has been reported to be an efficient hydroxylating biocatalyst. Previous works have shown that regardless of the bioreactor design or operation mode, MeOH concentration reaches a threshold after a few hours, but there are no investigations into the reasons behind this phenomenon. The present work entails monitoring both MeOH and formate concentrations during CH₄ hydroxylation, where neither a gaseous substrate nor nutrient shortage was evidenced. Under the assayed reaction conditions, bacterial stress was shown to occur, but methanol was not responsible for this. Formate additio

SUBMITTER: Baldo H 

PROVIDER: S-EPMC11381948 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature