Intra-patient spatial comparison of non-metastatic and metastatic lymph nodes reveals the reduction of CD169⁺ macrophages by metastatic breast cancers.

ABSTRACT:

Background

Breast cancer cells suppress the host immune system to efficiently invade the lymph nodes; however, the underlying mechanism remains incompletely understood. Here, we aimed to comprehensively characterise the effects of breast cancers on immune cells in the lymph nodes.

Methods

We collected non-metastatic and metastatic lymph node samples from 6 patients with breast cancer with lymph node metastasis. We performed bulk transcriptomics, spatial transcriptomics, and imaging mass cytometry to analyse the obtained lymph nodes. Furthermore, we conducted histological analyses against a larger patient cohort (474 slices from 58 patients).

Findings

The comparison between paired lymph nodes with and without metastasis from the same patients demonstrated that the numb

SUBMITTER: Maeshima Y 

PROVIDER: S-EPMC11382037 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature