MicroRNA-650 promotes melanoma metastasis via targeting inhibitor of growth family member 4.

ABSTRACT:

Objective

This study aimed to evaluate the effects of microRNA-650 (miR-650) on melanoma metastasis and reveal the regulatory relationship between miR-650 and the inhibitor of growth family member 4 (ING4).

Methods

miR-650 expression was determined in human melanoma WM115 and A-375 cells. WM115 cells were transfected with miR-650 mimic or mimic control. The invasion and migration abilities of transfected WM115 cells were analyzed using Transwell and wound healing assays, respectively. Then, miR-650-overexpression lentivirus vector was constructed and transfected into WM115 cells. After injection into the mice, the number of micro-metastatic foci in the lung tissues was counted. A regulatory relationship between miR-650 and ING4 was identified in WM115 and A-375 cells.

Res

SUBMITTER: Chen C 

PROVIDER: S-EPMC11382039 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature