Conformational Landscape of NADH and Ion Binding in Water/DMSO Mixtures via ³¹P NMR Spectroscopy.

ABSTRACT: This study reports on the conformational states of nicotinamide adenine dinucleotide (NADH) in water/DMSO mixtures and examines the influence of ion binding. We observe evidence of conformational changes through a series of NMR techniques, including ³¹P NMR relaxation (R₁ and R₂), chemical exchange saturation transfer (CEST), and diffusion-ordered spectroscopy (DOSY) experiments. The observed variation of the conformational states is indicative of the solvent's influence on NADH's structural flexibility. The experimental findings, in combination with viscosity data, are shown to be in line with findings from earlier molecular dynamics studies. The reported observations emphasize the critical role of the solvent environment in determining the c

SUBMITTER: Lu J 

PROVIDER: S-EPMC11382270 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature