Applications of Lightsheet Fluorescence Microscopy by High Numerical Aperture Detection Lens.

ABSTRACT: This Review explores the evolution, improvements, and recent applications of Light Sheet Fluorescence Microscopy (LSFM) in biological research using a high numerical aperture detection objective (lens) for imaging subcellular structures. The Review begins with an overview of the development of LSFM, tracing its evolution from its inception to its current state and emphasizing key milestones and technological advancements over the years. Subsequently, we will discuss various improvements of LSFM techniques, covering advancements in hardware such as illumination strategies, optical designs, and sample preparation methods that have enhanced imaging capabilities and resolution. The advancements in data acquisition and processing are also included, which provides a brief overview of the recent

SUBMITTER: Shih CP 

PROVIDER: S-EPMC11382282 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature