Screening of herbal extracts binding with vascular endothelial growth factor by applying HerboChip platform.

ABSTRACT:

Background

Traditional Chinese medicine (TCM) has been hailed as a rich source of medicine, but many types of herbs and their functions still need to be rapidly discovered and elucidated. HerboChip, a target-based drug screening platform, is an array of different fractions deriving from herbal extracts. This study was designed to identify effective components from TCM that interact with vascular endothelial growth factor (VEGF) as a target using HerboChip.

Methods

Selected TCMs that are traditionally used as remedies for cancer prevention and wound healing were determined and extracted with 50% ethanol. Biotinylated-VEGF was hybridized with over 500 chips coated with different HPLC-separated fractions from TCM extracts and straptavidin-Cy5 was applied to identify plant extra

SUBMITTER: Liu Y 

PROVIDER: S-EPMC11382504 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature