A non-tethering role for the Drosophila Pol θ linker domain in promoting damage resolution.

ABSTRACT: DNA polymerase theta ( Polθ ) is an error-prone translesion polymerase that becomes crucial for DNA double-strand break repair when cells are deficient in homologous recombination or non-homologous end joining. In some organisms, Polθ also promotes tolerance of DNA interstrand crosslinks. Due to its importance in DNA damage tolerance, Polθ is an emerging target for treatment of cancer and disease. Prior work has characterized the functions of the Polθ helicase-like and polymerase domains, but the roles of the linker domain are largely unknown. Here, we show that the Drosophila melanogaster Polθ linker domain promotes egg development and is required for tolerance of DNA double-strand breaks and interstrand crosslinks. While a linker domain with scrambled amino acid residues is suffic

SUBMITTER: Blanch JR 

PROVIDER: S-EPMC11383001 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature