RNA-Seq Analysis Unraveling Novel Genes and Pathways Influencing Corneal Wound Healing.

ABSTRACT:

Purpose

Transdifferentiation of corneal fibroblasts to myofibroblasts in the stroma is a central mechanistic event in corneal wound healing. This study sought to characterize genes and pathways influencing transdifferentiation of human corneal fibroblasts (hCSFs) to human corneal myofibroblasts (hCMFs) using RNA sequencing (RNA-seq) to develop comprehensive mechanistic information and identify newer targets for corneal fibrosis management.

Methods

Primary hCSFs were derived from donor human corneas. hCMFs were generated by treating primary hCSFs with transforming growth factor β1 (TGFβ1; 5 ng/mL) for 72 hours under serum-free conditions. RNA was extracted using the RNeasy Plus Mini Kit and subjected to RNA-seq analysis after quality control testing. Differential gene express

SUBMITTER: Kumar R 

PROVIDER: S-EPMC11383191 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature