Motor-driven microtubule diffusion in a photobleached dynamical coordinate system.

ABSTRACT: Motor-driven cytoskeletal remodeling in cellular systems can often be accompanied by a diffusive-like effect at local scales, but distinguishing the contributions of the ordering process, such as active contraction of a network, from this active diffusion is difficult to achieve. Using light-dimerizable kinesin motors to spatially control the formation and contraction of a microtubule network, we deliberately photobleach a grid pattern onto the filament network serving as a transient and dynamic coordinate system to observe the deformation and translation of the remaining fluorescent squares of microtubules. We find that the network contracts at a rate set by motor speed but is accompanied by a diffusive-like spread throughout the bulk of the contracting network with effective diffusion co

SUBMITTER: Hirokawa S 

PROVIDER: S-EPMC11383436 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature