Mosquitoes reared in distinct insectaries within an institution in close spatial proximity possess significantly divergent microbiomes.

ABSTRACT: The microbiome affects important aspects of mosquito biology and differences in microbial composition can affect the outcomes of laboratory studies. To determine how the biotic and abiotic conditions in an insectary affect the composition of the bacterial microbiome of mosquitoes we reared mosquitoes from a single cohort of eggs from one genetically homogeneous inbred Aedes aegypti colony, which were split into three batches, and transferred to each of three different insectaries located within the Liverpool School of Tropical Medicine. Using three replicate trays per insectary, we assessed and compared the bacterial microbiome composition as mosquitoes developed from these eggs. We also characterised the microbiome of the mosquitoes' food sources, measured environmental conditions

SUBMITTER: Brettell LE 

PROVIDER: S-EPMC11383675 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature