An alveolus lung-on-a-chip model of <i>Mycobacterium fortuitum</i> lung infection.

ABSTRACT: Lung disease due to non-tuberculous mycobacteria (NTM) is rising in incidence. While both two dimensional cell culture and animal models exist for NTM infections, a major knowledge gap is the early responses of human alveolar and innate immune cells to NTM within the human alveolar microenvironment. Here we describe development of a humanized, three-dimensional, alveolus lung-on-a-chip (ALoC) model of Mycobacterium fortuitum lung infection that incorporates only primary human cells such as pulmonary vascular endothelial cells in a vascular channel, and type I and II alveolar cells and monocyte-derived macrophages in an alveolar channel along an air-liquid interface. M. fortuitum introduced into the alveolar channel primarily infected macrophages, with rare bacteria inside alv

SUBMITTER: Ektnitphong V 

PROVIDER: S-EPMC11383683 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature