Self-Assembled Generation of Multi-zonal Liver Organoids from Human Pluripotent Stem Cells.

ABSTRACT: Distinct hepatocyte subpopulations are spatially segregated along the portal-central axis and critical to understanding metabolic homeostasis and liver injury. While several bioactive molecules have been described to play a role in directing zonal fates, including ascorbate and bilirubin, in vitro replication of zonal liver architecture has not been achieved to date. In order to evaluate hepatic zonal polarity, we developed a self-assembling zone-specific liver organoid culture by co-culturing ascorbate and bilirubin enriched hepatic progenitors derived from human induced pluripotent stem cells. We found that preconditioned hepatocyte-like cells exhibited zone-specific functions associated with urea cycle, glutathione synthesis and glutamate synthesis. Single nucleus RNA sequencing

SUBMITTER: Al Reza H 

PROVIDER: S-EPMC11384014 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature