Defining spatial nonuniformities of all ipRGC types using an improved Opn4^cre recombinase mouse line.

ABSTRACT: Intrinsically photosensitive retinal ganglion cells (ipRGCs) play a crucial role in several physiological light responses. In this study, we generate an improved Opn4^cre knockin allele (Opn4^cre(DSO)), which faithfully reproduces endogenous Opn4 expression and improves compatibility with widely used reporters. We evaluated the efficacy and sensitivity of Opn4^cre(DSO) for labeling in retina and brain and provide an in-depth comparison with the extensively utilized Opn4^cre(Saha) line. Through this characterization, Opn4^cre(DSO) demonstrated higher specificity in labeling ipRGCs with minimal recombination escape. Leveraging a combination of electrophysiological, molecular, and morphological analyses, we confirmed its sensitivity in detecti

SUBMITTER: Dyer B 

PROVIDER: S-EPMC11384080 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature