A chemoenzymatic method for simultaneous profiling N- and O-glycans on glycoproteins using one-pot format.

ABSTRACT: Glycosylation is generally characterized and controlled as a critical quality attribute for therapeutic glycoproteins because glycans can impact protein drug-product efficacy, half-life, stability, and safety. Analytical procedures to characterize N-glycans are relatively well established, but the characterization of O-glycans is challenging due to the complex workflows and lack of enzymatic tools. Here, we present a simplified chemoenzymatic method to simultaneously profile N- and O-glycans from the same sample using a one-pot format by mass spectrometry (MS). N-glycans were first released by PNGase F, followed by O-glycopeptide generation by proteinase K, selective N-glycan reduction, and O-glycan release by β-elimination during permethylation of both N- and O-glycans. Glycan structural

SUBMITTER: Ortega-Rodriguez U 

PROVIDER: S-EPMC11384086 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature