Simulating the Fluorescence of Di-8-ANEPPS in Solvents of Different Polarity.

ABSTRACT: Voltage-sensitive fluorescent probes, such as di-8-amino-naphthyl-ethylene-pyridinium-propyl-sulfonate (di-8-ANEPPS), are extremely useful for monitoring the membrane potential in biological and biophysical studies. However, because di-8-ANEPPS is very sensitive to its environment, it can be difficult to distinguish the degree to which a given external factor affects the observed fluorescence. Molecular dynamics simulations based on detailed atomic models make it possible to examine the particular characteristics of the system and predict the effects of the surroundings. Here, the sensitivity of the spectra of di-8-ANEPPS to solvent polarity is investigated by modeling the electronic transition between the ground and excited states using classical molecular mechanical force fields. The abs

SUBMITTER: Youngworth R 

PROVIDER: S-EPMC11384272 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature