Optical microsensing reveals spatiotemporal oxygen dynamics in cornea wounds that affect healing via reactive oxygen species.

ABSTRACT: Oxygen (O₂) metabolism plays a critical role in cornea wound healing, regeneration, and homeostasis; however, the underlying spatiotemporal mechanisms are poorly understood. Here we used an optical sensor to profile O₂ flux in intact and wounded corneas of mouse eyes. Intact corneas have unique centrifugal O₂ influx profiles, smallest flux at the cornea center, and highest at the limbus. Following cornea injury, the O₂ influx profile presents three distinct consecutive phases: a "decreasing" phase from 0 to 6 h, a "recovering" phase from 12 to 48 h, and a 'peak' phase from 48 to 72 h, congruent to previously described healing phases. Immediately after wounding, the O₂ influx drops at wound center and wound edge but does not change sign

SUBMITTER: Ma L 

PROVIDER: S-EPMC11384276 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature