Primary and Metastatic Pancreatic Cancer Cells Exhibit Differential Migratory Potentials.

ABSTRACT:

Objectives

Pancreatic ductal adenocarcinoma (PDAC) is characterized by early metastatic spread in more than 50% of patients. In this study, we sought to understand the migratory properties of (non)metastatic PDAC cells and determine whether the migration of cancer stem cell (CSC) populations accounts for the aggressive nature of this disease.

Methods

The migratory abilities of primary and metastatic PDAC cell lines were investigated using a microfluidic device and time-lapse photography. The velocity, time of delay of mobilization, and number of migratory cells were analyzed. Cancer stem cell subpopulations were isolated by fluorescence-activated cell sorting and their migratory properties compared with their non-CSC counterparts.

Results

Primary cancer cells exhibite

SUBMITTER: Park JK 

PROVIDER: S-EPMC11384603 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature