Red blood cell metabolomics identify ergothioneine as a key metabolite in DMARD-naive rheumatoid arthritis and response to methotrexate.

ABSTRACT: Using a new red blood cell (RBC) metabolite extraction protocol, we performed a metabolomic analysis on RBCs in rheumatoid arthritis (RA) patients treated or not with methotrexate (MTX), with the two following objectives: to compare the RBC metabolic profiles of MTX-naïve RA patients and healthy controls (HC), and to investigate whether RBC profiles before and after MTX treatment in RA differed between responders and non-responders. Plasma analysis was performed in parallel. Metabolites were extracted and identified in RBCs and plasma by liquid chromatography-mass spectrometry. We compared the metabolomic fingerprints of 31 DMARD-naïve RA patients and 39 HCs. We also compared the RBC and plasma metabolomes of 25 RA patients who responded or not to MTX therapy before (M0) and after a 3-mont

SUBMITTER: Sigaux J 

PROVIDER: S-EPMC11384731 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature