Unveiling impaired vascular function and cellular heterogeneity in diabetic donor-derived vascular organoids.

ABSTRACT: Vascular organoids (VOs), derived from induced pluripotent stem cells (iPSCs), hold promise as in vitro disease models and drug screening platforms. However, their ability to faithfully recapitulate human vascular disease and cellular composition remains unclear. In this study, we demonstrate that VOs derived from iPSCs of donors with diabetes (DB-VOs) exhibit impaired vascular function compared to non-diabetic VOs (ND-VOs). DB-VOs display elevated levels of reactive oxygen species (ROS), heightened mitochondrial content and activity, increased proinflammatory cytokines, and reduced blood perfusion recovery in vivo. Through comprehensive single-cell RNA sequencing, we uncover molecular and functional differences, as well as signaling networks, between vascular cell types and clusters withi

SUBMITTER: Naderi-Meshkin H 

PROVIDER: S-EPMC11384901 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature