Decoding the mannose receptor-mAb interaction: the importance of high-mannose N-glycans and glycan-pairing.

ABSTRACT: During the development process of therapeutic monoclonal antibodies (mAbs), it is crucial to control (critical) quality attributes such as N-glycosylation influencing pharmacokinetics (PK) and Fc effector functions. Previous reports have shown that mAbs containing high-mannose N-glycans are cleared faster from blood circulation, leading to reduced half-lives. The high-mannose N-glycan content of mAbs can be influenced during the cell culture process by factors such as cell lines, process conditions, and media. Furthermore, mAbs have either one high mannose N-glycan (asymmetrical high-mannose glyco-pair) or two high mannose N-glycans (symmetrical high-mannose glyco-pair). The hypothesis that the mannose receptor (MR, CD206) accelerates clearance by facilitating their internalization and sub

SUBMITTER: Baumeister J 

PROVIDER: S-EPMC11385167 | biostudies-literature | 2024 Jan-Dec

REPOSITORIES: biostudies-literature