Enhanced visualization of nuclear staining and cell cycle analysis for the human commensal Malassezia.

ABSTRACT: Malassezia is a lipophilic commensal yeast that resides mainly on the mammalian skin and is also found to associate with the internal organs. Dysbiosis of Malassezia is related to several diseases and often escapes detection as it is difficult to culture and maintain. Malassezia cell wall differs from other budding yeasts like S. cerevisiae due to the difference in the lipid content and is difficult to transform. In this study, we present a methodology to stain Malassezia's nucleus and perform cell cycle studies. However, staining presents a challenge due to its exceptionally thick cell wall with high lipid content, hindering conventional methods. Our novel methodology addresses this challenge and enables the staining of the Malassezia nucleus with a low background. This would allow resear

SUBMITTER: Sasikumar J 

PROVIDER: S-EPMC11385215 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature