The role of chromatin modulator DPY30 in glucose metabolism of colorectal cancer cells.

ABSTRACT:

Background

Colorectal cancer (CRC) is the third most common cancer worldwide and the second leading cause of cancer-related death. This study investigated the role of DPY30 in the development and progression of CRC cells, especially in the area of cellular glycolysis.

Methods

HT29 control cells and DPY30 knockdown cells were collected for tandem mass tag (TMT) labeling quantitative proteomics analysis of cellular total proteins (n=3). To further assess the accuracy of the differential expression profile, representative genes were selected and confirmed by quantitative real-time polymerase chain reaction (qPCR) and western blot (WB). Glycolytic flux was studied by detecting the extracellular acidification rate (ECAR) using the Seahorse XFe96. In view of the vital role of DPY30

SUBMITTER: Mao X 

PROVIDER: S-EPMC11385247 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature