A flow cytometry method for quantitative measurement and molecular investigation of the adhesion of bacteria to yeast cells.

ABSTRACT: The study of microorganism interactions is important for understanding the organization and functioning of microbial consortia. Additionally, the interaction between yeast and bacteria is of interest in the field of health and nutrition area for the development of probiotics. To investigate these microbial interactions at the cellular and molecular levels, a simple, reliable, and quantitative method is proposed. We demonstrated that flow cytometry enables the measurement of interactions at a single-cell level by detecting and counting yeast cells with bound fluorescent bacteria. Imaging flow cytometry revealed that the number of bacteria attached to yeast followed a Gaussian distribution whose maximum reached 14 bacterial cells using a clinical Escherichia coli strain E22 and the laborator

SUBMITTER: Schiavone M 

PROVIDER: S-EPMC11385505 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature