QTL-Seq identified a genomic region on chromosome 1 for soil-salinity tolerance in F₂ progeny of Thai salt-tolerant rice donor line "Jao Khao".

ABSTRACT:

Introduction

Owing to advances in high-throughput genome sequencing, QTL-Seq mapping of salt tolerance traits is a major platform for identifying soil-salinity tolerance QTLs to accelerate marker-assisted selection for salt-tolerant rice varieties. We performed QTL-BSA-Seq in the seedling stage of rice from a genetic cross of the extreme salt-sensitive variety, IR29, and "Jao Khao" (JK), a Thai salt-tolerant variety.

Methods

A total of 462 F₂ progeny grown in soil and treated with 160 mM NaCl were used as the QTL mapping population. Two high- and low-bulk sets, based on cell membrane stability (CMS) and tiller number at the recovery stage (TN), were equally sampled. The genomes of each pool were sequenced, and statistical significance of QTL was calculated using Q

SUBMITTER: Khunsanit P 

PROVIDER: S-EPMC11385611 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature