Reprogramming macrophages with R848-loaded artificial protocells to modulate skin and skeletal wound healing.

ABSTRACT: After tissue injury, inflammatory cells are rapidly recruited to the wound where they clear microbes and other debris, and coordinate the behaviour of other cell lineages at the repair site in both positive and negative ways. In this study, we take advantage of the translucency and genetic tractability of zebrafish to evaluate the feasibility of reprogramming innate immune cells in vivo with cargo-loaded protocells and investigate how this alters the inflammatory response in the context of skin and skeletal repair. Using live imaging, we show that protocells loaded with R848 cargo (which targets TLR7 and TLR8 signalling), are engulfed by macrophages resulting in their switching to a pro-inflammatory phenotype and altering their regulation of angiogenesis, collagen deposition and re-epithel

SUBMITTER: Lopez-Cuevas P 

PROVIDER: S-EPMC11385641 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature