Characteristics and transcriptional regulators of spontaneous epithelial-mesenchymal transition in genetically unperturbed patient-derived non-spindled breast carcinoma.

ABSTRACT:

Background

Although tumor cells undergoing epithelial-mesenchymal transition (EMT) typically exhibit spindle morphology in experimental models, such histomorphological evidence of EMT has predominantly been observed in rare primary spindle carcinomas. The characteristics and transcriptional regulators of spontaneous EMT in genetically unperturbed non-spindled carcinomas remain underexplored.

Methods

We used primary culture combined with RNA sequencing (RNA-seq), single-cell RNA-seq (scRNA-seq), and in situ RNA-seq to explore the characteristics and transcription factors (TFs) associated with potential spontaneous EMT in non-spindled breast carcinoma.

Results

Our primary culture revealed carcinoma cells expressing diverse epithelial-mesenchymal traits, consistent with

SUBMITTER: Lien HC 

PROVIDER: S-EPMC11385830 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature