Mesenchymal stem cell cryopreservation with cavitation-mediated trehalose treatment.

ABSTRACT: Dimethylsulfoxide (DMSO) has conventionally been used for cell cryopreservation both in research and in clinical applications, but has long-term cytotoxic effects. Trehalose, a natural disaccharide, has been proposed as a non-toxic cryoprotectant. However, the lack of specific cell membrane transporter receptors inhibits transmembrane transport and severely limits its cryoprotective capability. This research presents a method to successfully deliver trehalose into mesenchymal stem cells (MSCs) using ultrasound in the presence of microbubbles. The optimised trehalose concentration was shown to be able to not only preserve membrane integrity and cell viability but also the multipotency of MSCs, which are essential for stem cell therapy. Confocal imaging revealed that rhodamine-labelled treha

SUBMITTER: Fuenteslopez CV 

PROVIDER: S-EPMC11385975 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature