Individual methanogenic granules are whole-ecosystem replicates with reproducible responses to environmental cues.

ABSTRACT:

Background

In this study, individual methanogenic (anaerobic), granular biofilms were used as true community replicates to assess whole-microbial-community responses to environmental cues. The aggregates were sourced from a lab-scale, engineered, biological wastewater treatment system, were size-separated, and the largest granules were individually subjected to controlled environmental cues in micro-batch reactors (μBRs).

Results

Individual granules were identical with respect to the structure of the active community based on cDNA analysis. Additionally, it was observed that the active microbial community of individual granules, at the depth of 16S rRNA gene sequencing, produced reproducible responses to environmental changes in pH, temperature, substrate, and trace-metal su

SUBMITTER: Trego A 

PROVIDER: S-EPMC11386378 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature