2-Bromopalmitate treatment attenuates senescence phenotype in human adult cells - possible role of palmitoylation.

ABSTRACT: Cells may undergo senescence in response to DNA damage, which is associated with cell cycle arrest, altered gene expression and altered cell morphology. Protein palmitoylation is one of the mechanisms by which the DNA damage response is regulated. Therefore, we hypothesized that protein palmitoylation played a role in regulation of the senescent phenotype. Here, we showed that treatment of senescent human vascular smooth muscle cells (VSMCs) with 2-bromopalmitate (2-BP), an inhibitor of protein acyltransferases, is associated with changes in different aspects of the senescent phenotype, including the resumption of cell proliferation, a decrease in DNA damage markers and the downregulation of senescence-associated β-galactosidase activity. The effects were dose dependent and associated with

SUBMITTER: Krzystyniak A 

PROVIDER: S-EPMC11386925 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature