PKM2 regulates metabolic flux and oxidative stress in the murine heart.

ABSTRACT: Cardiac metabolism ensures a continuous ATP supply, primarily using fatty acids in a healthy state and favoring glucose in pathological conditions. Pyruvate kinase muscle (PKM) controls the final step of glycolysis, with PKM1 being the main isoform in the heart. PKM2, elevated in various heart diseases, has been suggested to play a protective role in cardiac stress, but its function in basal cardiac metabolism remains unclear. We examined hearts from global PKM2 knockout (PKM2^-/-) mice and found reduced intracellular glucose. Isotopic tracing of U-¹³C glucose revealed a shift to biosynthetic pathways in PKM2^-/- cardiomyocytes. Total ATP content was two-thirds lower in PKM2^-/- hearts, and functional analysis indicated reduced mitochondrial oxygen

SUBMITTER: Lee KCY 

PROVIDER: S-EPMC11387154 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature