A Carrier-Based Quantitative Proteomics Method Applied to Biomarker Discovery in Pericardial Fluid.

ABSTRACT: Data-dependent liquid chromatography tandem mass spectrometry is challenged by the large concentration range of proteins in plasma and related fluids. We adapted the SCoPE method from single-cell proteomics to pericardial fluid, where a myocardial tissue carrier was used to aid protein quantification. The carrier proteome and patient samples were labeled with distinct isobaric labels, which allowed separate quantification. Undepleted pericardial fluid from patients with type 2 diabetes mellitus and/or heart failure undergoing heart surgery was analyzed with either a traditional liquid chromatography tandem mass spectrometry method or with the carrier proteome. In total, 1398 proteins were quantified with a carrier, compared to 265 without, and a higher proportion of these proteins were of

SUBMITTER: Campbell AJ 

PROVIDER: S-EPMC11387241 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature