The septin cytoskeleton is required for plasma membrane repair.

ABSTRACT: Plasma membrane repair is a fundamental homeostatic process of eukaryotic cells. Here, we report a new function for the conserved cytoskeletal proteins known as septins in the repair of cells perforated by pore-forming toxins or mechanical disruption. Using a silencing RNA screen, we identified known repair factors (e.g. annexin A2, ANXA2) and novel factors such as septin 7 (SEPT7) that is essential for septin assembly. Upon plasma membrane injury, the septin cytoskeleton is extensively redistributed to form submembranous domains arranged as knob and loop structures containing F-actin, myosin IIA, S100A11, and ANXA2. Formation of these domains is Ca²⁺-dependent and correlates with plasma membrane repair efficiency. Super-resolution microscopy revealed that septins and F-actin fo

SUBMITTER: Prislusky MI 

PROVIDER: S-EPMC11387490 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature