Defining the functional properties of cyclopropane fatty acid synthase from Pseudomonas aeruginosa PAO1.

ABSTRACT: Cyclopropane fatty acid synthases (CFAS) catalyze the conversion of unsaturated fatty acids to cyclopropane fatty acids (CFAs) within bacterial membranes. This modification alters the biophysical properties of membranes and has been correlated with virulence in several human pathogens. Despite the central role played by CFAS enzymes in regulating bacterial stress responses, the mechanistic properties of the CFAS enzyme family and the consequences of CFA biosynthesis remain largely uncharacterized in most bacteria. We report the first characterization of the CFAS enzyme from Pseudomonas aeruginosa (PA), an opportunistic human pathogen with complex membrane biology that is frequently associated with antimicrobial resistance and high tolerance to various external stressors. We demonstrate tha

SUBMITTER: Ezeduru V 

PROVIDER: S-EPMC11387697 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature