A novel pan-proteome array for high-throughput profiling of the humoral response to Treponema pallidum.

ABSTRACT: Given the resurgence of syphilis, research endeavors to improve current assays for serological diagnosis and management of this disease are a priority. A proteome-scale platform for high-throughput profiling of the humoral response to Treponema pallidum (T. pallidum) proteins during infection could identify antigens suitable to ameliorate the performance and capabilities of treponemal tests for syphilis. Additionally, because infection-induced immunity is partially protective, profiling the response to T. pallidum outer membrane proteins (OMPs) could help select vaccine candidates. Therefore, we developed a pan-proteome array (PPA) based on the Nichols and SS14 strain complete proteomes and used it to define the immunoglobulin M (IgM) and IgG humoral response to T. 

SUBMITTER: Campo JJ 

PROVIDER: S-EPMC11387709 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature