Sendai virus-mediated RNA delivery restores fertility to congenital and chemotherapy-induced infertile female mice.

ABSTRACT: Current infertility treatment strategies focus on mature gametes, leaving a significant proportion of cases with gamete progenitors that stopped complete differentiation. On the other hand, recent advancements in next-generation sequencing have identified many candidate genes that may promote maturation of germ cells. Although gene therapy has shown success in mice, concerns about the integration of DNA vectors into oocytes hinder clinical applications. Here, we present the restoration of fertility in female mice through Sendai virus (SeV)-mediated RNA delivery. Ovaries lacking Kitl expression exhibit only primordial follicles due to impaired signaling to oocytes expressing the KIT tyrosine kinase. Despite SeVs being immunogenic and larger than the blood-follicle barrier, the admini

SUBMITTER: Kanatsu-Shinohara M 

PROVIDER: S-EPMC11388103 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature