Unveiling axolotl transcriptome for tissue regeneration with high-resolution annotation via long-read sequencing.

ABSTRACT: Axolotls are known for their remarkable regeneration ability. Exploring their transcriptome provides insight into regenerative mechanisms. However, the current annotation of the axolotl transcriptome is limited, leaving the role of unannotated transcripts in regeneration unknown. To discourse this challenge, we exploited long-read sequencing technology, which enables direct observation of full-length RNA transcripts, greatly enhancing the coverage and accuracy of axolotl transcriptome annotation. By utilizing this method, we identified 222 novel gene loci and 4775 novel transcripts, which were quantified using short-read sequencing data. Through the inclusive analysis, we discovered novel homologs, potential functional proteins, noncoding RNAs, and alternative splicing events in key regene

SUBMITTER: Qin T 

PROVIDER: S-EPMC11388199 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature