Altered memory CCR6⁺ Th17-polarised T-cell function and biology in people with HIV under successful antiretroviral therapy and HIV elite controllers.

ABSTRACT:

Background

Despite successful antiretroviral therapy (ART), frequencies and immunological functions of memory CCR6⁺ Th17-polarised CD4⁺ T-cells are not fully restored in people with HIV (PWH). Moreover, long-lived Th17 cells contribute to HIV persistence under ART. However, the molecular mechanisms underlying these observations remain understudied.

Methods

mRNA-sequencing was performed using Illumina technology on freshly FACS-sorted memory CCR6⁺CD4⁺ T-cells from successfully ART-treated (ST), elite controllers (EC), and uninfected donors (HD). Gene expression validation was performed by RT-PCR, flow cytometry, and in vitro functional assays.

Findings

Decreased Th17 cell frequencies in STs and ECs versus HDs coincided with r

SUBMITTER: Yero A 

PROVIDER: S-EPMC11388266 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature