Selective optogenetic inhibition of Gα_q or Gα_i signaling by minimal RGS domains disrupts circuit functionality and circuit formation.

ABSTRACT: Optogenetic techniques provide genetically targeted, spatially and temporally precise approaches to correlate cellular activities and physiological outcomes. In the nervous system, G protein-coupled receptors (GPCRs) have essential neuromodulatory functions through binding extracellular ligands to induce intracellular signaling cascades. In this work, we develop and validate an optogenetic tool that disrupts Gα_q signaling through membrane recruitment of a minimal regulator of G protein signaling (RGS) domain. This approach, Photo-induced Gα Modulator-Inhibition of Gα_q (PiGM-Iq), exhibited potent and selective inhibition of Gα_q signaling. Using PiGM-Iq we alter the behavior of Caenorhabditis elegans and Drosophila with outcomes consistent with

SUBMITTER: Lockyer JL 

PROVIDER: S-EPMC11388284 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature